Protocol detail

Lung and MLEC Protein Analyses

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Lung or MLEC protein analyses were performed as previously described (7 (link)) using LC3B, p62, Caspase3, autophagocytosis-associated protein 3 (ATG3), ATG7, Beclin-1 (Cell Signaling Technology), PINK1 (Millipore, clone N4/15), LAMP2A, mitofusin-1 (MFN1), MFN2, optic atrophy type 1 (OPA1), dynamin-related protein 1 (DRP1) (Abcam), Proteasome 20S α6 subunit (Enzo Life Sci), PARIS (Millipore, clone N196/16), Caspase 1, IL-1β, Parkin, PGC-1α and β-actin (Santa Cruz Biotechnology) antibodies.

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Zhang Y., Sauler M., Shinn A.S., Gong H., Haslip M., Shan P., Mannam P, & Lee P.J. (2014). Endothelial PINK1 mediates the protective effects of NLRP3 deficiency during lethal oxidant injury. Journal of immunology (Baltimore, Md. : 1950), 192(11), 5296-5304.

Publication 2014

Caspase3 Beclin-1 mitofusin-1 PARIS Caspase 1 IL-1β Parkin PGC-1α β-actin

Actin Antibodies Autophagocytosis Beclin 1 Caspase 1 Caspase3 Clone Dynamin related protein 1 Il 1β Lung Mfn2 Opa1 Optic atrophy type 1 Parkin Pgc 1α Proteasome Protein Subunit

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Variable analysis

independent variables

Lung or MLEC protein analyses

dependent variables

Levels of LC3B, p62, Caspase3, ATG3, ATG7, Beclin-1, PINK1, LAMP2A, MFN1, MFN2, OPA1, DRP1, Proteasome 20S α6 subunit, PARIS, Caspase 1, IL-1β, Parkin, PGC-1α and β-actin proteins

control variables

Experimental protocols used in the previous study (7 (link))

controls

Positive controls not specified
Negative controls not specified

Annotations

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