Protocol detail

Drosophila Genetic Stocks for Wnt Signaling

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The following stocks were used in this study: wg^CX4 b¹ pr¹/CyO (#2980), Wnt4^C1/CyO (#6651), Wnt2^L/CyO (#6909), Wnt5^Gal4 (#59034), Df(1)N19/FM6 (#970), Df(2L)ED729/SM6a (#24134) daughterless::Gal4 (#5460) (Bloomington Drosophila Stock Center, Bloomington, USA; stock numbers given in parentheses). P(GSV3)GS8107 (#201394, Kyoto Drosophila Stock Center); fz^J22, fz^P21 (gifts from Paul Adler, University of Virginia, Charlottesville, USA), fz^R52 (gift from Ken Cadigan, University of Michigan, Ann Arbor, USA); fz2^C2 (gift from G. Struhl, Columbia University, New York City, USA); Df(3L)469-2 (Bhanot et al., 1999 (link)); Wnt5⁴⁰⁰ (Fradkin et al., 2004 (link)); otk^A1, otk2^C26, Df(otk,otk2)D72 (Linnemannstöns et al., 2014 (link)); Transgenic fly lines for the constructs UAS::Ror-Myc and Ror::Ror-eGFP were generated as described (Bischof et al., 2007 (link); Fish et al., 2007 (link)).

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Ripp C., Loth J., Petrova I., Linnemannstöns K., Ulepic M., Fradkin L., Noordermeer J, & Wodarz A. (2018). Drosophila Ror is a nervous system-specific co-receptor for Wnt ligands. Biology Open, 7(11), bio033001.

Publication 2018

) daughterless::Gal4

Drosophila Fish Gifts Transgenic

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Variable analysis

independent variables

Wg^CX4 b^1 pr^1/CyO
Wnt4^C1/CyO
Wnt2^L/CyO
Wnt5^Gal4
Df(1)N19/FM6
Df(2L)ED729/SM6a
Daughterless::Gal4
P(GSV3)GS8107
Fz^J22
Fz^P21
Fz^R52
Fz2^C2
Df(3L)469-2
Wnt5^400
Otk^A1
Otk2^C26
Df(otk,otk2)D72
UAS::Ror-Myc
Ror::Ror-eGFP

dependent variables

Not explicitly mentioned

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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