IHC for p53 (1:1,000, clone DO-7, DAKO, Santa Clara, CA, USA) and PTEN (1:1,000, clone Y184, Abcam, Cambridge, UK) was performed on 3-μm-thick sections from the TMA block using a Ventana automated immunostainer (BenchMark XT, Ventana Medical Systems, Tucson, AZ, USA), in accordance with the manufacturer's instructions. IHC was interpreted by intensity (negative, weak, and strong) and area (%). The PTEN and p53 expression were classified into three categories: (1) strong staining in ≥10% of the tumor cells was considered as strong positive, (2) samples without any nuclear staining of tumor cells were interpreted as negative, and (3) samples exhibiting weak staining in any area or patchy strong staining in <10% of tumor cells were regarded as weakly positive [28 (link)].
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Immunohistochemical Analysis of p53 and PTEN
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Other organizations : Seoul National University Bundang Hospital, Seoul National University Hospital
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Variable analysis
independent variables
- IHC for p53 (1:1,000, clone DO-7, DAKO, Santa Clara, CA, USA)
- IHC for PTEN (1:1,000, clone Y184, Abcam, Cambridge, UK)
- PTEN and p53 expression classified into three categories: (1) strong staining in ≥10% of the tumor cells, (2) samples without any nuclear staining of tumor cells, and (3) samples exhibiting weak staining in any area or patchy strong staining in <10% of tumor cells
- 3-μm-thick sections from the TMA block
- Ventana automated immunostainer (BenchMark XT, Ventana Medical Systems, Tucson, AZ, USA)
- Manufacturer's instructions
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