Transcription Factors and Chromatin Regulators Identification

We also adjusted the identification method of transcription co-factors and CRFs. First, we extracted both of them for human from Tcof-DB (31 (link)) and GO database by related GO items. For transcription co-factors, the used GO items are ‘transcription coactivator activity’, ‘transcription corepressor activity’, ‘transcription co-factor activity’ and ‘regulation of transcription’. For CRFs, the GO annotations are ‘chromatin remodeling’, ‘chromatin-mediated maintenance of transcription’, ‘histone *ylation’, ‘histone .*ylase activity’ and ‘histone *transferase activity’. After manual curation and removing redundant genes, 415 transcription co-factors and 142 CRFs were obtained in human genome. To identify them in other 64 species, we did the reciprocal best-hits Basic Local Alignment Search Tool (BLAST) between human and other species with the threshold setting as E-value ≤ 1e-4, coverage ≥ 50% and identity ≥ 30%.