Rainbow trout (Oncorhynchus mykiss) (30 ± 5 g) and grass carp (Ctenopharyngodon idella) (200 ± 20 g) were purchased from Dujiang Dam Rainbow Trout Farm (Chengdu, China) and Xiantao Hatchery (Xiantao, China) respectively. They were maintained and acclimated to the laboratory conditions for at least two weeks before experiments. All animal experiments in this study were approved by the Committee on the Ethics of Animal Experiments at Huazhong Agricultural University.
Human embryonic kidney 293T (HEK293T) cells were cultured in 5% CO2 at 37°C. Dulbecco’s modified Eagle’s medium (DMEM) (HyClone) was supplemented with 10% fetal bovine serum (FBS) (Gibco), 100 g/mL penicillin (Sigma-Aldrich), and 100 g/mL streptomycin (Sigma-Aldrich).
Human embryonic kidney 293T (HEK293T) cells were cultured in 5% CO2 at 37°C. Dulbecco’s modified Eagle’s medium (DMEM) (HyClone) was supplemented with 10% fetal bovine serum (FBS) (Gibco), 100 g/mL penicillin (Sigma-Aldrich), and 100 g/mL streptomycin (Sigma-Aldrich).
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