Docetaxel and CAPE Cytotoxicity Assay

PC/DX25 and DU/DX50 cells were seeded at a density of 3 × 10³ cells/well in 96-well plates with 100 μl RPMI-1640 medium containing 10% FBS and increasing concentration of docetaxel and CAPE for 96 h. Relative cell number was analyzed by measuring the DNA content of cell lysates with Hoechst dye 33,258-based 96-well proliferation assay (Sigma, St. Louis, MO, USA) as described previously [17 (link)]. All readouts were normalized to the average of the control condition in each individual experiment. The experiment was repeated at least eight times.

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Fu Y.K., Wang B.J., Tseng J.C., Huang S.H., Lin C.Y., Kuo Y.Y., Hour T.C, & Chuu C.P. (2022). Combination treatment of docetaxel with caffeic acid phenethyl ester suppresses the survival and the proliferation of docetaxel-resistant prostate cancer cells via induction of apoptosis and metabolism interference. Journal of Biomedical Science, 29, 16.

Publication 2022

Hoechst dye 33,258-based 96-well proliferation assay

Assay Docetaxel

Corresponding Organization : China Medical University

Other organizations : National Health Research Institutes, Kaohsiung Medical University

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Variable analysis

independent variables

Docetaxel concentration
CAPE concentration

dependent variables

Relative cell number

control variables

Cell line (PC/DX25 and DU/DX50)
Cell seeding density (3 × 10^3 cells/well)
Plate type (96-well)
Culture medium (RPMI-1640 with 10% FBS)
Culture duration (96 h)

controls

Control condition (no treatment)

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