Levofloxacin microfiber sutures were manufactured using the wet electrospinning setup depicted in Figure 1A and described by Zhang and coworkers.37 (link) Briefly, PLLA (221 kDa; Corbion, Amsterdam, Netherlands) at 86% to 89% (wt/wt) was mixed with Levofloxacin (Sigma Aldrich, St. Louis, MO) at 10 wt% and either PEG (35 kDa; Sigma Aldrich) or Pluronic F127 (BASF, Florham Park, NJ) between 1 and 4 wt% and dissolved in chloroform (Sigma Aldrich) at room temperature for 24 hours. Levofloxacin concentration was held constant and PLLA concentration in chloroform was maintained at 15 wt% in all formulations. Sutures were produced by wet electrospinning the polymer/drug solution in a setup consisting of a high voltage power supply (Gamma High Voltage Research, Ormond Beach, FL), syringe pump (Fisher Scientific, Waltham, MA), and rotating metal collector with hexane (Sigma Aldrich) as the lending solvent. The polymer solution was ejected through a blunted 18-G needle (Fisher Scientific) at 13 mL/h with 4.7 kV of applied voltage 5 cm away from the collector rotating at 40 rpm. Fibers were then collected and desiccated for 2 days prior to storage at −20°C.