Inhibition of Allergen-Specific IgE by CCD

1:20 Anti-CCD absorbent (EUROIMMUN Medizinische Labordiagnostika AG, Lübeck, SH, Germany) was added to the samples to be tested, and then mixed well and incubated at room temperature (18–25°C) in a shaker for 1 h. Immediately afterward, sIgE of mugwort and allergen components (Art v 1, Art v 3 and Art v 4), ragweed and allergen components (Amb a 1), bermuda grass and allergen components (Cyn d 1 and Cyn d 12), and timothy grass and allergen components (Phl p 1, Phl p 4, Phl p 5, Phl p 7, and Phl p 12), and CCD were tested via the EUROBlotMaster system. The inhibition rates of CCD on pollen allergens were calculated by (sIgE after CCD inhibition minus the corresponding values before CCD inhibition and then divide by before levels) (20 (link), 21 (link)).