Conditioned Medium Cytokine Analysis

Fifth-passage RIFs were cultured in α-MEM (without phenol red; Gibco) without FBS for 24 hours. Supernatants were collected and filtered with MILLEX-GP 0.22-μm filters (Merck Millipore Ltd. Co., Cork, Munster, Ireland) to remove cell debris and concentrated to 1% volume with Amicon Ultra-4 centrifugal ultrafiltration tubes (NMWL 3KDa) (Merck Millipore Ltd. Co.) for use as conditioned medium (CM).
The concentrations of IL-1β, IL-6, TNF-α and activated TGF-β1 in CM was detected by ELISA kits (R&D Systems Inc., Minneapolis, MN, USA) according to the manufacturer’s instructions and assessed by densitometry analysis as previously described^{56 (link)}.

Free full text: Click here

Jin J., Tao J., Gu X., Yu Z., Wang R., Zuo G., Li Q., Lv X, & Miao D. (2017). P16INK4a Deletion Ameliorated Renal Tubulointerstitial Injury in a Stress-induced Premature Senescence Model of Bmi-1 Deficiency. Scientific Reports, 7, 7502.

Publication 2017

α-MEM (without phenol red; MILLEX-GP 0.22-μm filters Amicon Ultra-4 centrifugal ultrafiltration tubes (NMWL 3KDa)

Cell Conditioned medium Densitometry Elisa Il 1β Rifs Tgf β1 Tnf α Ultrafiltration

Corresponding Organization :

Other organizations : Nanjing Medical University, Jiangsu Vocational College of Medicine

Top 5 similar protocols

Protocol cited in 2 other protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Concentrations of IL-1β, IL-6, TNF-α and activated TGF-β1 in the conditioned medium (CM)

control variables

Fifth-passage RIFs were cultured in α-MEM (without phenol red; Gibco) without FBS for 24 hours
Supernatants were collected and filtered with MILLEX-GP 0.22-μm filters (Merck Millipore Ltd. Co., Cork, Munster, Ireland) to remove cell debris
Supernatants were concentrated to 1% volume with Amicon Ultra-4 centrifugal ultrafiltration tubes (NMWL 3KDa) (Merck Millipore Ltd. Co.) for use as conditioned medium (CM)

controls

No positive or negative controls were explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!