Antibody Characterization for LRIG1 Analysis

The antigen peptides for all LRIG1 antibodies used are indicated in Supplementary Fig. 1B. For immunohistochemistry: Anti-LRIG1 antibody (Atlas Antibodies AB, Bromma, Sweden, catalog no. HPA011846), 4.0 mg/ml. The 1A8 anti-LRIG1 antibody is described in the Supplementary Materials. For western blots: EGFR #2232, 1:1000; Phospho-p44/42 MAPK #4370, 1:1000; Phospho-AKT #4060, 1:1000 (Cell Signaling Technology Inc, Danvers, USA); Anti-Actin #ACTN05/C4, 1:3000 (Abcam, Cambridge, UK). Anti-LRIG1 Vina [49 (link)], 1:1000 (Agrisera AB, Vännäs, Sweden). Secondary antibodies: IRDye^® 680RD Donkey anti-Mouse #926-68072, 1:15000; IRDye^® 800CW Goat anti-Rabbit #926-32211, 1:15000 (LI-COR, Lincoln, NE, USA).