Glucose-Stimulated C-Peptide Secretion Assay

The IPCs were assessed the function by glucose-stimulated C-peptide secretion assay (GSCS)^{24 (link),38 (link),69 (link),70 (link),138 (link)}. The IPCs were incubated in normal KRH bicarbonate (KRBH) buffer (pH 7.4) for 1 h as basal control, then in 5.5 mM of glucose anhydrous (Sigma) in KRBH for the next 1 h and finally in 22 mM glucose anhydrous in KRBH for 1 h. Enzyme-linked immunosorbent assay (ELISA) kit (Millipore) was used for detecting the generated C-peptide level according to the manufacturer’s protocol. Total DNA (ng) and stimulation time (mins) were used to normalization.

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Dang Le Q., Rodprasert W., Kuncorojakti S., Pavasant P., Osathanon T, & Sawangmake C. (2022). In vitro generation of transplantable insulin-producing cells from canine adipose-derived mesenchymal stem cells. Scientific Reports, 12, 9127.

Publication 2022

glucose anhydrous Enzyme-linked immunosorbent assay (ELISA) kit

Assay Bicarbonate Buffer C peptide Enzyme linked immunosorbent assay Glucose Secretion

Corresponding Organization :

Other organizations : Chulalongkorn University

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Variable analysis

independent variables

Glucose concentration (5.5 mM and 22 mM)

dependent variables

C-peptide secretion

control variables

Normal KRH bicarbonate (KRBH) buffer (pH 7.4)
Incubation time (1 hour for basal control, 1 hour for 5.5 mM glucose, 1 hour for 22 mM glucose)
Total DNA (ng) for normalization
Stimulation time (mins) for normalization

controls

Positive control: 22 mM glucose stimulation
Negative control: Basal incubation in normal KRBH buffer

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