Antibody Profiling for Herpes Virus Infection

Primary rabbit antibodies included anti-actin (Sigma-Aldrich; A5060), anti-enhanced green fluorescent protein (anti-eGFP; Abcam; ab290), anti-Daxx (Upstate; 07-471), anti-PIAS1 (LsBio; LS-B9173), anti-PIAS2 (LsBio; LS-C108717), anti-PIAS3 (LsBio; LS-C98795), anti-PIAS4 (LsBio; LS-C108719), anti-PML (Bethyl Laboratories; A301-167A), anti-Sp100 (SpGH [56 (link)]), anti-SUMO1 (Abcam; ab32058), and anti-SUMO2/3 (Abcam; ab22654) antibodies. Primary mouse monoclonal antibodies included anti-His (Abcam; ab18184), anti-ICP0 (11060 [57 (link)]), anti-ICP4 (58s [58 (link)]), anti-SUMO1 (Invitrogen; 33-2400), anti-SUMO2/3 (Abcam; ab81371), anti-UL42 (Z1F11 [59 (link)]), and anti-VP5 (DM165 [60 (link)]) antibodies. Secondary antibodies included DyLight 680- or 800-conjugated anti-rabbit or -mouse (Thermo) antibodies; Alexa 488-, 555-, or 633-conjugated anti-rabbit, -sheep, or -mouse (Invitrogen) antibodies; and peroxidase-conjugated anti-mouse antibodies (Sigma-Aldrich; A4416).

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Conn K.L., Wasson P., McFarlane S., Tong L., Brown J.R., Grant K.G., Domingues P, & Boutell C. (2016). Novel Role for Protein Inhibitor of Activated STAT 4 (PIAS4) in the Restriction of Herpes Simplex Virus 1 by the Cellular Intrinsic Antiviral Immune Response. Journal of Virology, 90(9), 4807-4826.

Publication 2016

ab32058 ab18184 ab81371

A301 Actin Anti antibodies Antibodies Daxx Enhanced green fluorescent protein Monoclonal antibodies Mouse Peroxidase Pias1 Pias3 Rabbit Sheep Sp100 Sumo1

Corresponding Organization :

Other organizations : MRC University of Glasgow Centre for Virus Research

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Variable analysis

independent variables

Primary rabbit antibodies
Primary mouse monoclonal antibodies

dependent variables

Not explicitly mentioned

control variables

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controls

Positive controls: None specified
Negative controls: None specified

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