Validated microRNAs were purchased as pre-miRNAs from Ambion (Grand Island, New York), and used with the recommended Ambion pre-miRNA control. Anti-miRs and a scrambled Anti-miR control for validated oncogenic miRNAs were also purchased from Ambion. miRNA transfections were carried out using Oligofectamine (Invitrogen, Grand Island, New York) reagent as previously described [36] (link).
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