Immunohistochemical Profiling of Breast Cancer

For immunohistochemistry (IHC), antibodies specific for ER (1:100 clone 6F11; Novocastra, Newcastle upon Tyne, UK), progesterone receptor (PR; clone 16; Novocastra), HER2 (4B5 rabbit monoclonal antibody; Ventana Medical Systems, Tucson, AZ, USA), and Ki-67 (MIB-1; Dako, Glostrup, Denmark) were stained using formalin-fixed, paraffin-embedded tissue sections [17 (link)]. Positivity of ER and PR IHC expression was defined according to the modified Allred system: positive, Allred score 2–8; and negative, Allred score 0–1 [18 (link)]. All tumors included in this study had ER positivity (Allred scores ≥3). HER2 status was considered positive with a score of 3+ and negative with a score of 0 or 1+ [19 (link)]. Tumors with a score of 2+ underwent fluorescent in situ hybridization analysis according to the manufacturer’s instructions (PathVysion kit; Vysis, Downers Grove, IL, USA or HER2 inform; Ventana) [19 (link)]. Ki-67 expression was evaluated by YJC and displayed presented as a percentage (range 0–100%) of positive tumor cells.

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Ahn S.G., Cha Y.J., Bae S.J., Yoon C., Lee H.W, & Jeong J. (2018). Comparisons of tumor-infiltrating lymphocyte levels and the 21-gene recurrence score in ER-positive/HER2-negative breast cancer. BMC Cancer, 18, 320.

Publication 2018

clone 6F11 Ki-67 (MIB-1; HER2 inform

Antibodies Cells Clone Fluorescent in situ hybridization Formalin Her2 Immunohistochemistry Mib 1 Monoclonal antibody Paraffin Progesterone receptor Rabbit Tissue Tumor

Corresponding Organization : Yonsei University

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Variable analysis

independent variables

Antibody type (ER, PR, HER2, Ki-67)

dependent variables

ER and PR IHC expression (Allred score)
HER2 status (score 0, 1+, 2+, 3+)
Ki-67 expression (percentage of positive tumor cells, range 0-100%)

control variables

Formalin-fixed, paraffin-embedded tissue sections
All tumors included had ER positivity (Allred scores ≥3)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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