The GFP (1-248)-tagged full-length human MxA was a gift from Dr. Jovan Pavlovic (University of Zurich, Zurich, Switzerland) [17 (link)]. Transient transfections were carried out using just subconfluent cultures in 35 mm plates using DNA in the range of 0.3–2 µg/culture and the Polyfect reagent (Qiagen, Germantown, MD, USA) and the manufacturer’s protocol (with 10 µL Polyfect reagent per 35 mm plate) [19 (link)].
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