Radiolabeled Transporter Assay Protocol

³H-MPP (1-Methyl-4-phenylpyridinium iodide) and ¹⁴C-metformin were purchased from American Radiolabeled Chemicals Saint louis; Missouri, USA. All non labelled chemicals were obtained from Sigma-Aldrich, Darmstadt, Germany. For transfection, the following cDNAs were used: hOCT1 (GeneBank: accession number: NM_003057.2), mOct1 (NM_009202.5), rOct1 (NM_012697.1), hMATE1 (NM_018242.2), and hOCT2 (NM_003058.3). The hOCT1 cloned has the genotype Ser14, Arg61, Cys88, Phe160, Gly401,Met408, Met420 and Gly465, which corresponds to the OCT1*1B allele according to the nomenclature suggested by Seitz et al. [29 (link)].

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Floerl S., Kuehne A, & Hagos Y. (2020). Functional and Pharmacological Comparison of Human, Mouse, and Rat Organic Cation Transporter 1 toward Drug and Pesticide Interaction. International Journal of Molecular Sciences, 21(18), 6871.

Publication 2020

3H-MPP 14C-metformin

1 methyl 4 phenylpyridinium Allele Cdnas Genotype Iodide Metformin Oct1 Transfection

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Variable analysis

independent variables

³H-MPP (1-Methyl-4-phenylpyridinium iodide)
¹⁴C-metformin

dependent variables

Not explicitly mentioned

control variables

All non labelled chemicals were obtained from Sigma-Aldrich, Darmstadt, Germany
The hOCT1 cloned has the genotype Ser14, Arg61, Cys88, Phe160, Gly401,Met408, Met420 and Gly465, which corresponds to the OCT1*1B allele according to the nomenclature suggested by Seitz et al. [29]

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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