Fly Triglyceride Measurement Protocol

Total triglycerides normalized to total protein were measured as described in (14 (link)). Briefly, two flies per biological replicate were homogenized in lysis buffer [140 mM NaCl, 50 mM tris-HCl (pH 7.4), and 0.1% Triton X-100] containing protease inhibitor cocktail (Thermo Fisher Scientific). Lysate extract was used to determine protein and triglyceride concentrations using Pierce bicinchoninic acid (BCA) assay (Thermo Fisher Scientific; absorbance, 562 nm) and Triglycerides LiquiColor Test (Stanbio; abs, 500 nm), respectively.

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Vaziri A., Khabiri M., Genaw B.T., May C.E., Freddolino P.L, & Dus M. (2020). Persistent epigenetic reprogramming of sweet taste by diet. Science Advances, 6(46), eabc8492.

Publication 2020

protease inhibitor cocktail Pierce bicinchoninic acid (BCA) assay Triglycerides LiquiColor Test

Assay Bicinchoninic acid Biological Buffer Flies Nacl Protease inhibitor Protein Replicate Triglycerides Tris Triton x 100

Corresponding Organization :

Other organizations : University of Michigan–Ann Arbor

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Total triglycerides normalized to total protein

control variables

Lysis buffer composition (140 mM NaCl, 50 mM tris-HCl (pH 7.4), and 0.1% Triton X-100)
Protease inhibitor cocktail (Thermo Fisher Scientific)

controls

Positive control: None mentioned
Negative control: None mentioned

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