H. hepaticus 3B1 (ATCC 51449) were purchased from ATCC (Maryland, USA), and were cultured on Brucella agar plates (BD, USA) supplemented with 5% defibrinated sheep blood and antibiotics for 4–5 days under microaerobic conditions (85% N2, 10% CO2, 5% O2) at 37°C. Bacteria were harvested in PBS and used for oral infection when OD600 reading was 1.15 (link)
Protocol detail
Culturing H. hepaticus for Oral Infection
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : Yangzhou University, Jiangnan University, Jiangsu Institute of Parasitic Diseases
Top 5 similar protocols
Variable analysis
independent variables
- Oral infection with H. hepaticus 3B1 (ATCC 51449)
- Not explicitly mentioned
- Culture of H. hepaticus 3B1 (ATCC 51449) on Brucella agar plates supplemented with 5% defibrinated sheep blood and antibiotics
- Microaerobic conditions (85% N2, 10% CO2, 5% O2) at 37°C
- Positive control: Not specified
- Negative control: Not specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!