16S rRNA Sequencing of Colon Microbiome
Corresponding Organization :
Other organizations : Hunan Agricultural University
Variable analysis
- Method of 16S rRNA sequencing in colon microbes
- Colon microbiome composition
- Genomic DNA quality control
- Primer splice design and synthesis
- Polymerase chain reaction (PCR) amplification
- PCR product purification
- Quantification
- Homogenization
- MiSeq high-throughput sequencing (Illumina, USA)
- No positive or negative controls were explicitly mentioned.
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