Quantifying Beclin1 and KOR Silencing

ON-TARGET plus SMART Pool Human Beclin1 siRNA (L-010552-00) and human KOR siRNA (L-005684-00) were used as per the manufacturer's instructions. Transfections were carried out as previously described.^{41 (link)} Scrambled siRNA was used as a negative control. The reduction in Beclin1 and KOR mRNA expression were estimated by real-time PCR analysis. Caspase-3/7 activity was analyzed using Caspase-Glo luminescent-based assays according to the manufacturer's instructions (Promega Corporation).

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Ma J., Wan J., Meng J., Banerjee S., Ramakrishnan S, & Roy S. (2014). Methamphetamine induces autophagy as a pro-survival response against apoptotic endothelial cell death through the Kappa opioid receptor. Cell Death & Disease, 5(3), e1099-.

Publication 2014

Caspase-Glo luminescent-based assays

Beclin1 Caspase Caspase 7 Human Luminescent assays Mrna Promega Real time pcr analysis Sirna Transfections

Corresponding Organization :

Other organizations : University of Minnesota

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Variable analysis

independent variables

ON-TARGET plus SMART Pool Human Beclin1 siRNA (L-010552-00)
Human KOR siRNA (L-005684-00)

dependent variables

Beclin1 mRNA expression
KOR mRNA expression
Caspase-3/7 activity

control variables

Transfections were carried out as previously described

controls

Positive control: Not mentioned
Negative control: Scrambled siRNA

Annotations

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