DNA fragmentation due to shRNA treatment was studied using Apo-BrdU-Red In-situ DNA fragmentation assay kit (Biovision, California, USA). HSP70-2 shRNA3, shRNA4 and NC shRNA transfected MDA-MB-231 cells were processed as described earlier [13 (link)]. The cells were analyzed at 576 nm using BD-FACS VERSA. (BD Biosciences, California, USA).
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