Cell Cycle Analysis and Clonogenic Assay

MTT and Flow Cytometry assays were performed as previously described47 (link)54 (link). For Flow Cytometry assay, MCF-7 cells transfected with different plasmids were stained with propidium iodide (PI) (BD Pharmingen, CA). Experimental data were collected by FACSCalibur (BD Biosciences, San Jose, CA, USA). Cell cycle profiles were determined using ModFit LT (BD Biosciences). For crystal violet staining assay, MCF-7 cells transfected with the appropriate plasmids were transferred to 35 mm plate, and were cultured until the recognizable clones appeared. Then, the cells were stained with crystal violet for 30 min at room temperature.