Protocol detail

Liver Protein Extraction and Immunoblotting

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Protein was extracted from liver tissue or cell cultures, as described^{53 (link)}. Monoclonal rabbit anti-mouse ATF3 (Santa Cruz Biotechnology, Shanghai, China), phos-mTOR, mTOR, phos-p70S6K, p70S6K, HMGB1, TLR4, NF-κB, HIF-1α, PHD1, Bcl-2, Bcl-xl, and β-actin Abs (Cell Signaling Technology, San Diego, CA) were used. The relative quantities of proteins were determined by densitometer, and expressed in absorbance units (AU).

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Zhu Q., Wang H., Jiang B., Ni X., Jiang L., Li C., Wang X., Zhang F., Ke B, & Lu L. (2018). Loss of ATF3 exacerbates liver damage through the activation of mTOR/p70S6K/ HIF-1α signaling pathway in liver inflammatory injury. Cell Death & Disease, 9(9), 910.

Publication 2018

phos-mTOR phos-p70S6K p70S6K HMGB1 NF-κB HIF-1α Bcl-2 Bcl-xl β-actin Abs

Actin Bcl 2 Cell cultures Hmgb1 Mouse Mtor Nf κb P70s6k Phd1 Proteins Rabbit

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Variable analysis

independent variables

Protein extraction from liver tissue or cell cultures

dependent variables

Relative quantities of proteins determined by densitometer, expressed in absorbance units (AU)

control variables

Monoclonal rabbit anti-mouse ATF3 (Santa Cruz Biotechnology, Shanghai, China)
Phos-mTOR, mTOR, phos-p70S6K, p70S6K, HMGB1, TLR4, NF-κB, HIF-1α, PHD1, Bcl-2, Bcl-xl, and β-actin Abs (Cell Signaling Technology, San Diego, CA)

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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