Mass Spectrometric Identification of BioR Proteins

The identity of two versions of P. denitrificans BioR proteins (BioR1 and BioR2) was verified using A Waters Q-Tof API-US Quad-ToF mass spectrometer connected to a Waters nano Acquity UPLC (Feng and Cronan 2011 (link)). As we described before (Feng and Cronan 2011 (link)), the protein band of interest was digested with Trypsin (G-Biosciences St. Louis, MO), and the resultant peptides were loaded on a Waters Atlantis C-18 column (0.03 mm particle, 0.075 × 150 mm). The dependently acquired data were further subjected to the ms/ms analyses.

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Feng Y., Kumar R., Ravcheev D.A, & Zhang H. (2015). Paracoccus denitrificans possesses two BioR homologs having a role in regulation of biotin metabolism. MicrobiologyOpen, 4(4), 644-659.

Publication 2015

Q-Tof API-US Quad-ToF mass spectrometer nano Acquity UPLC Trypsin Atlantis C-18 column

Ms ms Peptides Protein Trypsin

Corresponding Organization :

Other organizations : Zhejiang University, Texas A&M Health Science Center, University of Luxembourg

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Variable analysis

independent variables

Waters Q-Tof API-US Quad-ToF mass spectrometer
Waters nano Acquity UPLC
Trypsin (G-Biosciences St. Louis, MO)
Waters Atlantis C-18 column (0.03 mm particle, 0.075 × 150 mm)

dependent variables

Identity of two versions of P. denitrificans BioR proteins (BioR1 and BioR2)

control variables

Not explicitly mentioned

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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