Antioxidant Enzyme Activity Assay

The 8-day-old seedlings grown on 0%, 0.5%, or 2% sucrose 1/2 MS medium were harvested and total proteins were extracted at 4 °C. Superoxide dismutase (SOD) and catalase (CAT) activities were measured with SOD and CAT assay kits (Comin Biotechnology Co., Ltd., Suzhou, China) as described [30 (link)]. A spectrophotometer (DU730, Beckman Coulter, Pasadena, CA, USA) was used to measure the absorbances at 560 nm and 240 nm for SOD and CAT, respectively.

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Yuan Y., Xu D., Xiang D., Jiang L, & Hu H. (2022). Serine Hydroxymethyltransferase 1 Is Essential for Primary-Root Growth at Low-Sucrose Conditions. International Journal of Molecular Sciences, 23(9), 4540.

Publication 2022

DU730

Assay Catalase Proteins Seedlings Sucrose Superoxide dismutase

Corresponding Organization : Huazhong Agricultural University

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Variable analysis

independent variables

Sucrose concentration (0%, 0.5%, or 2%) in 1/2 MS medium

dependent variables

Superoxide dismutase (SOD) activity
Catalase (CAT) activity

control variables

Age of seedlings (8 days old)
Temperature of protein extraction (4 °C)

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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