Immunoblotting Analysis Protocol

Standard protocols were used to perform all immunoblotting analyses (described in [13 (link), 24 (link)]). After incubation with appropriate secondary antibody, signals were detected using immuno-chemiluminescent reagents (GE Healthcare, Piscataway, NJ). β-actin antibody (#A300–491, Bethyl) was used as a protein loading control. See Additional file 2: Supplementary Methods for detailed protocol. All the antibodies used for immuno-blotting has been listed in the Additional file 2: Table S1.

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Chagani S., Wang R., Carpenter E.L., Löhr C.V., Ganguli-Indra G, & Indra A.K. (2017). Ablation of epidermal RXRα in cooperation with activated CDK4 and oncogenic NRAS generates spontaneous and acute neonatal UVB induced malignant metastatic melanomas. BMC Cancer, 17, 736.

Publication 2017

immuno-chemiluminescent reagents β-actin antibody

A protein Actin Antibodies Antibody

Corresponding Organization :

Other organizations : Oregon State University

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Signals detected using immuno-chemiluminescent reagents

control variables

β-actin antibody (#A300–491, Bethyl) used as a protein loading control

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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