FITC Dextran Absorption Assay

The FITC dextran assay was conducted as previously described [41 (link),42 (link)]. Briefly, food and water were removed from mice for 4 h followed by oral gavage with 500 mg/kg FITC dextran (Sigma Aldrich, St. Louis, MO, USA) reconstituted in sterile PBS. Mice were euthanized and blood collected via cardiac puncture. The FITC dextran levels in the serum were measured using a fluorimeter (Agilent BioTek Synergy LX, Santa Clara, CA, USA), and the concentration was calculated using an FITC standard curve.

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Nettleford S.K., Liao C., Short S.P., Rossi R.M., Singh V, & Prabhu K.S. (2023). Selenoprotein W Ameliorates Experimental Colitis and Promotes Intestinal Epithelial Repair. Antioxidants, 12(4), 850.

Publication 2023

FITC dextran Synergy LX

Assay Blood Cardiac Fitc Fitc dextran Food Gavage Mice Puncture Serum Sterile

Corresponding Organization : Pennsylvania State University

Other organizations : University of California, San Francisco, Vanderbilt University Medical Center

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Variable analysis

independent variables

Oral gavage with 500 mg/kg FITC dextran (Sigma Aldrich, St. Louis, MO, USA) reconstituted in sterile PBS

dependent variables

FITC dextran levels in the serum

control variables

Food and water were removed from mice for 4 h

controls

FITC standard curve was used to calculate the concentration of FITC dextran

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