About 3 cm of small bowel tissue above 2 cm from the ileocecal area was fixed with 10% formalin, paraffin embedded, and sectioned. After dewaxing with xylene, a series of ethanol was used for hydration. The sections were stained with hematoxylin (B600020; Proteintech, China) for 5 min. After washing with alkaline PBS and bluing for 2 min, they were rinsed with running water for 3 min. Afterwards, they were stained with eosin (Sigma, USA) for 10 min and flash washed with distilled water. Neutral gums were used for coverslipping. The morphological and structural changes of intestinal mucosal epithelium were observed under an optical microscope (eclipse Ci-e; Nikon, Japan). According to the results of hematoxylin and eosin staining, the degree of intestinal injury was evaluated by Chiu's score [15 (link)].
Free full text: Click here
