Synthesis of Amide Compound 135

Example 107

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Compound 135. The mixture of succinimidyl ester 129 (4.6 mg, 0.0068 mmol), DIEA (24 μL, 0.0138 mmol) and 1-hexadecylamine (21 mg, 0.0087 mmol) in DMF (1 mL) and CHCl₃ (2 mL) was stirred for 30 min, then diluted with chloroform (80 mL), washed with water (4×40 mL), brine 40 (mL), filtered through paper filter and evaporated. The residue was purified by chromatography on silica gel column (0.5×8 cm, packed with CHCl₃/MeOH/AcOH/H₂O (20:5:5:1)), eluant: CHCl₃/MeOH/AcOH/H₂O (20:5:5:1) to give amide 135 (4 mg, 73%) as a purple gum.