Characterization of Extracellular Vesicles by TEM
Corresponding Organization : Aarhus University
Variable analysis
- Mounting 5 μl of EV isolate on a grid
- Staining with 1% (w/v) phosphotungstic acid (pH 7.0)
- Incubating the grid with primary anti-CD9 antibody (1:50)
- Incubating the grid with secondary goat anti-mouse antibody conjugated with 10 nm colloidal gold (1:25)
- Presence of EV-specific marker CD9 on the surface of vesicles
- Phenotypic and structural characteristics of EVs observed by Transmission Electron Microscopy (TEM)
- Blocking the pelleted vesicles in ovalbumin
- Staining the grids with 1% (w/v) phosphotungstic acid at pH 7.0
- Blotting the grids dry
- Not explicitly mentioned
- Not explicitly mentioned
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