Reconstitution and Dilution of Cyclophosphamide and Bendamustine

Cyclophosphamide (Sigma-Aldrich, St. Louis, MO) and bendamustine (SelleckChem, Houston, TX) were reconstituted and diluted as described previously (2 (link)). Cyclophosphamide was reconstituted in ddH₂O to a stock concentration of 50 mg/mL then diluted with sterile saline (General Laboratory Products, Yorkville, IL) for i.p. injection. bendamustine was reconstituted in dimethyl sulfoxide (Sigma-Aldrich) to a stock concentration of 75 mg/mL, and diluted with sterile phosphate-buffered saline (GE Healthcare Life Sciences) containing 0.2% carboxymethylcellulose and 0.25% polysorbate 80 (Sigma-Aldrich) for i.v. injection.

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Molina M.S., Stokes J., Hoffman E.A., Eremija J., Zeng Y., Simpson R.J, & Katsanis E. (2020). Bendamustine Conditioning Skews Murine Host DCs Toward Pre-cDC1s and Reduces GvHD Independently of Batf3. Frontiers in Immunology, 11, 1410.

Publication 2020

Cyclophosphamide bendamustine dimethyl sulfoxide sterile phosphate-buffered saline polysorbate 80

Bendamustine Carboxymethylcellulose Cyclophosphamide Dimethyl sulfoxide Phosphate Polysorbate 80 Saline Sterile

Corresponding Organization : University of Arizona

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Variable analysis

independent variables

Cyclophosphamide
Bendamustine

dependent variables

Not explicitly mentioned

control variables

DdH2O
Sterile saline
Dimethyl sulfoxide
Sterile phosphate-buffered saline
Carboxymethylcellulose
Polysorbate 80

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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