We performed a cell-based coronavirus immunostaining assay using a rabbit (2019-nCoV) spike S1 antibody (1:50; Sino Biological, Duesseldorfer, Germany) and goat anti-rabbit secondary antibody conjugated with Alexa Fluor 647 (A-21245; 1:1000; Invitrogen, Carlsbad, CA, USA), as previously described (Stefanik et al., 2021 (link)).
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