48 h after the injection of 89Zr-OKT11 (anti-CD2)-F(ab´)2 or 89Zr-T3-3A1 (anti-CD7)-F(ab´)2, mice were anesthetized and imaging was performed with the Inveon small animal PET/CT scanner (Siemens, Knoxville, TN) as previously described 33 (link),34 (link). The CT scan was followed by 20-min static PET acquisition. A modified Feldcamp algorithm was used for reconstruction of CT images and 3-dimensional ordered subsets expectation maximum algorithm (OSEM3D/MAP) with a pixel size of 0.77 mm for the reconstruction of PET images. Fusion images were then created using the Inveon Research Workplace (Siemens, Knoxville, TN). Data were normalized and corrected for random, dead time, and decay with no correction for attenuation or scatter. To quantify tracer uptake, regions of interest were drawn based on the CT image and the activity accumulation referred as percentage of injected dose per gram of tissue (%ID/g, 1 cc = 1 g). Pictures are shown as 3D-maximum intensity projections (MIP) of co-registered PET/CT images.
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Quantitative PET Imaging of CD2 and CD7 Expression
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Corresponding Organization : German Cancer Research Center
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Variable analysis
independent variables
- Injection of 89Zr-OKT11 (anti-CD2)-F(ab´)2 or 89Zr-T3-3A1 (anti-CD7)-F(ab´)2
- Tracer uptake, referred as percentage of injected dose per gram of tissue (%ID/g)
- Anesthesia of mice
- Imaging with Inveon small animal PET/CT scanner
- CT scan followed by 20-min static PET acquisition
- Reconstruction of CT images using modified Feldcamp algorithm
- Reconstruction of PET images using 3-dimensional ordered subsets expectation maximum algorithm (OSEM3D/MAP)
- Normalization and correction of data for random, dead time, and decay (no correction for attenuation or scatter)
- No positive or negative controls were explicitly mentioned in the provided information.
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