Quantifying VSV Infection in BHK-21 Cells

BHK-21 cells (2.5 × 10⁴ cells) were infected with VSV at an MOI of 0.1 in the presence of various concentrations of each small molecule in a 96-well plate and incubated for 24 h as described in Section 2.4. The culture supernatants were subjected to a plaque assay to determine their virus titers [26 (link)]. Similarly, BHK-21 cells were infected with VSV-AcGFP and cultured for 24 h. After addition of Hoechst 33342 to culture medium to a final concentration of 5 µg/mL, cells were incubated for 30 min at 37 °C in a CO₂ incubator, and then observed under a fluorescent microscope with a 20× objective (EVOS FLoid Cell Imaging Station, Thermo Fisher Scientific).