Encapsulation of D-Limonene in Alginate-Gelatin Complex

D-Limonene, gelatin (type A with an isoelectric point of 7), n-hexane, pepsin, pancreatin 8XUSP were purchased from Sigma Aldrich (St. Louis, MO, United States). High viscosity sodium alginate (GRINDSTED Alginate FD 155) with pKa of 3.5 (Dupont Nutrition and Health, New Century, KS, United States) was selected for maximizing the extent of complex coacervation (16 , 24 (link)). Succinic acid, ammonium hydroxide, sodium hydroxide, sodium chloride and isopropanol were purchased from Fisher Scientific (Fair Lawn, NJ, United States). Anti-foam reagent was purchased from Spectrum Chemicals Mfg Corp (New Brunswick, NJ, United States). Aquacoat^® ECD 30 (aqueous colloidal dispersion of ethylcellulose polymer-30% ethylcellulose stabilized by sodium lauryl sulfate (SLS) and cetyl alcohol) was provided by Colorcon (Harleysville, PA, United States) and tributyl citrate was purchased from TCI America (Portland, OR, United States). Limonene, carvone and Limonene oxide standards were purchased from Sigma Aldrich (St. Louis, MO, United States).

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Tang Y., Park H., Scher H.B, & Jeoh T. (2022). The role of a moisture-barrier latex in controlling retention, stability and release of D-limonene from complex coacervated matrix microparticles formed during spray drying. Frontiers in Nutrition, 9, 979656.

Publication 2022

D-limonene gelatin (type A n-hexane pepsin pancreatin 8XUSP Succinic acid ammonium hydroxide sodium hydroxide sodium chloride isopropanol Limonene carvone limonene oxide

Alginate Ammonium hydroxide Aquacoat Carvone Cetyl alcohol Ethylcellulose Gelatin Isopropanol Limonene Limonene oxide N hexane Pancreatin Pepsin Polymer Sodium alginate Sodium chloride Sodium hydroxide Sodium lauryl sulfate Succinic acid Tributyl citrate Viscosity

Corresponding Organization : University of California, Davis

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Variable analysis

independent variables

D-limonene
Gelatin (type A with an isoelectric point of 7)
N-hexane
Pepsin
Pancreatin 8XUSP
High viscosity sodium alginate (GRINDSTED Alginate FD 155) with pKa of 3.5

dependent variables

Extent of complex coacervation

control variables

Succinic acid
Ammonium hydroxide
Sodium hydroxide
Sodium chloride
Isopropanol
Anti-foam reagent
Aquacoat® ECD 30 (aqueous colloidal dispersion of ethylcellulose polymer-30% ethylcellulose stabilized by sodium lauryl sulfate (SLS) and cetyl alcohol)
Tributyl citrate
Limonene, carvone and limonene oxide standards

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