Immunofluorescence Staining of CD11b, SPP1, and FABP4

The steps for immunofluorescence are the same as shown in our previous article [33 (link)]. The paraffin-embedded slides were incubated with mouse anti-human CD11b (ab212505, Abcam), rabbit anti-human SPP1 (ab214050, Abcam), or rabbit anti-human FABP4 (ab92501, Abcam) and then horseradish peroxidase-conjugated secondary antibody. After that, the slides were stained with DAPI.

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Hu Z., Jin X., Hong W., Sui Q., Zhao M., Huang Y., Li M., Wang Q., Zhan C, & Chen Z. (2023). Dissecting the single-cell transcriptome network of macrophage and identifies a signature to predict prognosis in lung adenocarcinoma. Cellular Oncology (Dordrecht).

Publication 2023

ab214050 ab92501

Antibody Cd11b Dapi Fabp4 Horseradish peroxidase Human Immunofluorescence Mouse Paraffin Rabbit Spp1

Corresponding Organization :

Other organizations : Zhongshan Hospital, Fudan University

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Variable analysis

independent variables

Mouse anti-human CD11b (ab212505, Abcam)
Rabbit anti-human SPP1 (ab214050, Abcam)
Rabbit anti-human FABP4 (ab92501, Abcam)

dependent variables

Immunofluorescence staining

control variables

Paraffin-embedded slides
Horseradish peroxidase-conjugated secondary antibody
DAPI staining

controls

No positive or negative controls were explicitly mentioned.

Annotations

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