Optokinetic Reflex Tracking Protocol

The methods for OKR recording to assess visual function were similar to prior studies (7 (link)). OKR experiments were performed using OptoMotry software and apparatus (Cerebral Mechanics Inc., Medicine Hat, AB, Canada). OKR function was determined by the highest spatial frequency at which mice track a 100% contrast grating projected at varying spatial frequencies, and data are reported as cycles/degree.

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Khan R.S., Dine K., Wessel H., Brown L, & Shindler K.S. (2019). Effects of Varying Intranasal Treatment Regimens in ST266-Mediated RGC Neuroprotection. Journal of neuro-ophthalmology : the official journal of the North American Neuro-Ophthalmology Society, 39(2), 191-199.

Publication 2019

OptoMotry software and apparatus

Mechanics Medicine Mice Visual function

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Variable analysis

independent variables

Spatial frequency of the grating projected

dependent variables

Highest spatial frequency at which mice track a 100% contrast grating

control variables

Contrast of the grating (100%)

controls

No positive or negative controls were explicitly mentioned.

Annotations

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