A total of 40 mice were randomly divided into four groups (10 mice per group) as follows: Control group (Control), MK-801 group (MK-801), MK-801 plus A2BAR agonist group (MK-801 + BAY) and MK-801 plus A2B AR antagonist group (MK-801 + PSB). The SCZ model was established according to a previously described method (26 (link)). Briefly, mice were injected intraperitoneally with MK-801 (0.6 mg/kg/day; cat. no. M107; MilliporeSigma) once a day for 14 consecutive days, and the intervention was performed between 3:00 and 4:00 p.m. every day. The control group was treated with the same volume of normal saline. The optimal dose of A2BAR selective agonist BAY 60-6583 (21 (link),27 (link),28 (link)) and A2B AR selective antagonist PSB 603 (21 (link),29 (link)) was determined according to previous studies. Intraperitoneal injection of BAY 60-6583 (80 µg/kg/day; cat. no. 910487-58-0; Tocris Bioscience) and PSB 603 (25 µg/kg/day; cat. no. 1092351-10-40; Tocris Bioscience) was administered to the MK-801 + BAY and MK-801+ PSB groups, respectively, every 2 days between day 8 and day 14 of the MK-801 treatment period. The mice in the control and MK-801 groups were intraperitoneally injected with an equal volume of normal saline.
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Modulation of Schizophrenia-like Behaviors
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Other organizations : Ningxia Medical University
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Variable analysis
independent variables
- MK-801 treatment (0.6 mg/kg/day for 14 days)
- BAY 60-6583 (A2BAR agonist) treatment (80 µg/kg/day, every 2 days from day 8 to day 14)
- PSB 603 (A2BAR antagonist) treatment (25 µg/kg/day, every 2 days from day 8 to day 14)
- Outcome measures not explicitly mentioned
- Treatment with normal saline (control group)
- Positive control: MK-801 group
- Negative control: Control group
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