Assessing MFB-F11 Cell Response

MFB‐F11 cells were cultured as described previously (Tesseur et al, 2006 (link)). In brief, 20 000 MFB‐F11 cells were seeded per well in 96‐well plate until 70% confluency. Cells were washed twice with 1× PBS, followed by incubation with medium from the co‐culture experiments for 24 h at 37°C, 5% CO₂. SEAP assay was carried out using Great EscAPe SEAP Chemiluminescence kit (Takara, 631738) according to the manufacturer’s instruction.

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Le H.Q., Hill M.A., Kollak I., Keck M., Schroeder V., Wirth J., Skronska‐Wasek W., Schruf E., Strobel B., Stahl H., Herrmann F.E., Campos A.R., Li J., Quast K., Knebel D., Viollet C., Thomas M.J., Lamb D, & Garnett J.P. (2021). An EZH2‐dependent transcriptional complex promotes aberrant epithelial remodelling after injury. EMBO Reports, 22(8), e52785.

Publication 2021

Great EscAPe SEAP Chemiluminescence kit

Assay Cells Chemiluminescence Culture medium

Corresponding Organization :

Other organizations : Boehringer Ingelheim (Germany), University of Bath, Discovery Institute, Sanford Burnham Prebys Medical Discovery Institute, Boehringer Ingelheim (United States), Newcastle University

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Variable analysis

independent variables

Medium from the co-culture experiments

dependent variables

SEAP activity

control variables

Cell density (20,000 MFB-F11 cells per well)
Cell culture conditions (37°C, 5% CO2)
Cell confluency (70%)
Washing cells with 1x PBS before treatment

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