Mycelia of F. graminearum cultured in PDB medium at 28°C with or without 100 μg/ml of crude methanol extract of BS45 for 48 h at 28°C. The cell suspension was mixed with 0.4% Trypan blue staining solution (Sangon Biotech Co., Ltd., Shanghai, China) in an appropriate proportion (0.04% final concentration of Trypan blue), stained for 3 min and observed under a light microscope (BM2000, Nanjing Jiangnan Novel Optics Co., Ltd., Nanjing).
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