The capture antibody was diluted to 1 mg/mL in 1 mM phosphate buffer.
The anti-IL-6 antibody (L152) was dispensed onto the NC membrane using
a BioDot (ZX1010) dispensing platform at a flow rate of 1 μL/cm
to obtain a test line width of 1 mm. Then, the NC membrane was dried
in an oven at 37 °C for 1 h. The simplified half dipstick LFD
was then assembled by placing the NC membrane on the backing card
followed by the absorbent pad with a 5 mm overlap on the NC membrane.
Finally, strips were cut 5 mm wide before use.
The anti-IL-6 antibody (L152) was dispensed onto the NC membrane using
a BioDot (ZX1010) dispensing platform at a flow rate of 1 μL/cm
to obtain a test line width of 1 mm. Then, the NC membrane was dried
in an oven at 37 °C for 1 h. The simplified half dipstick LFD
was then assembled by placing the NC membrane on the backing card
followed by the absorbent pad with a 5 mm overlap on the NC membrane.
Finally, strips were cut 5 mm wide before use.
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