The colonic segments were fixed in Methanol-Carnoy solution (methanol: chloroform: glacial acetic acid = 6:3:1), paraffin embedded and cut into serial 4 μm sections. Then, goblet cell staining was performed via Alcian blue/periodic acid-Schiff staining according to the instructions. The pathologist, without knowledge of any experimental procedures, counted the number of goblet cells.
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