Zebrafish embryos were amputated at the tail as previously described (Hale et al., 2017 (link)). Amputations were performed at 2 dpf and regeneration was analyzed at 5 dpf. Regenerated tails were imaged and whole embryos were lysed for genotyping. Regeneration was quantified by measuring the length from the tip of the notochord to the end of the fin fold in ImageJ.
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