iMSCs were generated by inducing hiPSCs into neural crest cells (NCCs), and then into iMSCs (Kamiya et al., 2022 (link)), which were then maintained in a plastic dish coated with Fibronectin (Chemicon, FC010-10 MG) and fed with PRIME-XV®MSC Expansion XSFM (IrvineScientific, 91149). Analyses were performed during passages 3—5.
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