Animals were used under the supervision of an approved institutional protocol. Adult female Yorkshire pigs (mean weight 30 kg) were purchased from E.M. Parsons and Sons (Hadley, MA). General anesthesia was induced with 4.4 mg/kg of intramuscular Telazol (Fort Dodge Labs, Fort Dodge, IA). Once sedated, animals were intubated with a cuffed endotracheal tube, and anesthesia was maintained with 2% isoflurane/balance O2. A lower midline abdominal incision was used to expose the uterus and fallopian tubes. A NIR fluorescent (800 nm emission) hysterosalpingogram was performed using 10 μM indocyanine green (ICG; Akorn, Decatur, IL) in saline injected in utero. NIR fluorescence (700 nm emission) angiography was performed by intravenous bolus injection of 1 mg/kg methylene blue (Akorn). Real-time NIR fluorescence imaging was performed as described in [4 (link)] except that each independent NIR fluorescence image, i.e., 700 nm emission and 800 nm emission, could be assigned different pseudo-colors from a multi-color palette, and could have its brightness, contrast, and gamma adjusted independently by the surgeon.