Innate Immunity and DNA Damage Response Pathway Analysis

Etoposide, 4-hydroxytamoxifen, puromycin, and polybrene were purchased from Sigma. Hygromycin was from Gemini Bio Products, and luciferin was from PerkinElmer. Recombinant human IL1α protein (used at 20 ng/ml) was from R&D Systems, and p38 MAPK inhibitor from Selleck Chemicals.
The following antibodies were described previously: Flag, p16, Lamin B1, GAPDH, and GFP^{5 (link)}. Other antibodies used include: γH2AX (Abcam #ab2893 and Cell Signaling Technology #9718), STING (Cell Signaling Technology #13647 and LSBio #LS-B7237), cGAS (Cell Signaling Technology #15102 and Santa Cruz Biotechnology #sc-245858), human IL1α (Abcam #ab9614), IL8 (Abcam #ab18672), p-ATM S1981 (Abcam #ab81292), p-p53 S15 (Cell Signaling Technology #9284), p-p65 S536 (Cell Signaling Technology #3033), p65 (Cell Signaling Technology 8242), NRas (Santa Cruz Biotechnology #sc-31), CD3 (Abcam #ab16669), Mac2 (BioLegend #125401), mouse IL1α (R&D Systems #AF-400), p21 (#sc-471), and H3 (Active Motif #39763).

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Dou Z., Ghosh K., Vizioli M.G., Zhu J., Sen P., Wangensteen K.J., Simithy J., Lan Y., Lin Y., Zhou Z., Capell B.C., Xu C., Xu M., Kieckhaefer J.E., Jiang T., Shoshkes-Carmel M., Ahasan Al Tanim K.M., Barber G.N., Seykora J.T., Millar S.E., Kaestner K.H., Garcia B.A., Adams P.D, & Berger S.L. (2017). Cytoplasmic chromatin triggers inflammation in senescence and cancer. Nature, 550(7676), 402-406.

Publication 2017

Etoposide 4-hydroxytamoxifen puromycin polybrene Hygromycin luciferin Recombinant human IL1α protein p38 MAPK inhibitor γH2AX STING p-ATM S1981 p-p53 S15 p-p65 S536 ab16669 AF-400

4 hydroxytamoxifen Antibodies Cgas Etoposide Gapdh Human Hygromycin Il1α Lamin b1 Luciferin Mouse Nras P38 mapk Polybrene Puromycin Recombinant protein

Corresponding Organization :

Other organizations : University of Pennsylvania, Cancer Research UK Scotland Institute, Peking University, Sylvester Comprehensive Cancer Center, University of Miami, University of Glasgow

Top 5 similar protocols

Variable analysis

independent variables

Etoposide
4-hydroxytamoxifen
Puromycin
Polybrene
Hygromycin
Luciferin
Recombinant human IL1α protein (used at 20 ng/ml)
P38 MAPK inhibitor

dependent variables

γH2AX
STING
Human IL1α
P-ATM S1981
P-p53 S15
P-p65 S536
Mouse IL1α

control variables

Lamin B1
GAPDH

Annotations

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