MHCC-97H cell were treated with indicated concentrations of anti-tumor agents and analyzed by transwell assays performed in 24-well plates chamber (Cat. No.: Costar 3422, Corning, Lowell, MA, USA) fitted with a polyethylene terephthalate filter membrane with 8-μm pores. The invasion-transwell or migration-transwell was performed following the methods described by Ji et al. and Liang et al.34 (link), 35 (link).
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