Lipopolysaccharide Extraction and Phage Inactivation

LPS was extracted from A. xylosoxidans using an LPS extraction kit from Intron Biotechnology (17144; Boca Scientific, Boca Raton, FL, USA), according to the manufacturer’s instructions. A control containing LPS from Escherichia coli O111:B4, purchased from Sigma-Aldrich, Inc. (L2630; Sigma, USA), was used as the negative control to ensure that any effect was specific to A. xylosoxidans LPS. Phage inactivation by LPS was tested as previously described24 (link).

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Li E., Yin Z., Ma Y., Li H., Lin W., Wei X., Zhao R., Jiang A., Yuan J, & Zhao X. (2016). Identification and molecular characterization of bacteriophage phiAxp-2 of Achromobacter xylosoxidans. Scientific Reports, 6, 34300.

Publication 2016

LPS extraction kit

Boca Escherichia coli Intron Phage

Corresponding Organization :

Other organizations : South China Agricultural University, Henan Institute of Science and Technology, Academy of Military Medical Sciences

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Variable analysis

independent variables

LPS extraction from A. xylosoxidans using an LPS extraction kit from Intron Biotechnology

dependent variables

Phage inactivation by LPS

control variables

LPS from Escherichia coli O111:B4, purchased from Sigma-Aldrich, Inc. (L2630; Sigma, USA) as a negative control

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