Diabetes was induced by a single intraperitoneal injection of streptozotocin (Sigma, St. Louis, MO, USA) at a dose of 40 mg/kg [18 (link)19 (link)]. streptozotocin was freshly prepared in 10 mM citrate buffer (pH 4.6), and non-diabetic control animals were injected with citrate buffer only. Blood glucose concentrations were monitored using an Accu-Chek® Active (Roche Diagnostics GmbH, Mannheim, Germany) glucose meter and diabetes mellitus was defined as hyperglycemic conditions with a glucose concentration exceeding 300 mg/dL. The body weight was decreased dose- and time dependent after streptozotocin injection [20 21 (link)].
Protocol detail
Streptozotocin-Induced Diabetic Rat Model
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Animals
Blood glucose
Body weight
Buffer
Citrate
Diabetes mellitus
Diagnostics
Glucose
Hyperglycemic conditions
Intraperitoneal injection
Streptozotocin
Corresponding Organization : Gyeongsang National University
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Variable analysis
independent variables
- Streptozotocin dose (40 mg/kg)
- Blood glucose concentration
- Body weight
- Citrate buffer (pH 4.6)
- Positive control: Streptozotocin-induced diabetic animals
- Negative control: Non-diabetic animals injected with citrate buffer only
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