Dendritic Morphology Analysis of CA1 Pyramidal Neurons

To be included in the analysis, a loaded neuron had to satisfy the following criteria: (1) reside within the pyramidal layer of the CA1 as defined by cytoarchitectural characteristics; (2) demonstrate complete filling of dendritic tree, as evidenced by well-defined endings; and (3) demonstrate intact tertiary branches, with the exception of branches that extended beyond 50 μm in radial distance from the cell soma
[75 (link), 76 (link), 78 (link)]. Neurons meeting these criteria were reconstructed in 3-dimensions (3D) with a 40×/1.4 N.A., Plan-Apochromat oil immersion objective on a Zeiss Axiophot 2 microscope equipped with a motorized stage, video camera system, and Neurolucida morphometry software (MBF Bioscience). Using NeuroExplorer software (MBF Bioscience) total dendritic length, number of intersections, and the amount of dendritic material per radial distance from the soma, in 30-μm increments
[79 (link)] were analyzed in order to assess morphological cellular diversity and potential differences between the animal groups.

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Price K.A., Varghese M., Sowa A., Yuk F., Brautigam H., Ehrlich M.E, & Dickstein D.L. (2014). Altered synaptic structure in the hippocampus in a mouse model of Alzheimer’s disease with soluble amyloid-β oligomers and no plaque pathology. Molecular Neurodegeneration, 9, 41.

Publication 2014

Axiophot 2 microscope Neurolucida morphometry software NeuroExplorer software

Animal Cell soma Cellular Dendritic Immersion Intersections Microscope Neurons Radial Tree

Corresponding Organization :

Other organizations : Icahn School of Medicine at Mount Sinai, Allen Institute for Brain Science

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Protocol cited in 2 other protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Total dendritic length
Number of intersections
Amount of dendritic material per radial distance from the soma, in 30-μm increments

control variables

Neurons that reside within the pyramidal layer of the CA1 as defined by cytoarchitectural characteristics
Neurons that demonstrate complete filling of dendritic tree, as evidenced by well-defined endings
Neurons that demonstrate intact tertiary branches, with the exception of branches that extended beyond 50 μm in radial distance from the cell soma

controls

Positive control: None mentioned
Negative control: None mentioned

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